Rift Valley Region comprises of the dioceses of Nakuru, Kitui, Machakos and the Archdiocese of Nairobi.
The current Regional Superior is Sr. Agnes Njeri (Nairobi).
The Sisters in this region are to be found in the following SSJ houses.
- Eastleigh B - St Theresa's Parish
- Eastleigh C - St. Theresas Parish Dispensary Convent
- St. Joseph Nursing Home, Eastleigh
- Villa Joseph Convent - Langata
- St. Anuarite Hostel Convent, CUEA Langata
- Katoloni Convent, Machakos
- Makueni Convent
- Thatha Convent, Machakos
- Ikanga Convent, Kitui
- St. Joseph the Worker Convent, Nakuru
Nairobi Region Sisters' Conference