Rift Valley Region comprises of the dioceses of Nakuru, Kitui, Machakos and the Archdiocese of Nairobi.
The current Regional Superior is Sr. Regina Nthenya (Nairobi).
The Sisters in this region are to be found in the following SSJ houses.
- Eastleigh B - St Theresa's Parish
- Eastleigh C - St. Theresas Parish Dispensary Convent
- St. Joseph Nursing Home, Eastleigh
- Villa Joseph Convent - Langata
- Ongata Rongai Community, Ngong
- Katoloni Convent, Machakos
- Makueni Convent
- Thatha Convent, Machakos
- Ikanga Convent, Kitui
- St. Joseph the Worker Convent, Nakuru
- Muranga Diocese
- Gilgil Community
- Kitale Community
Nairobi Region Sisters' Conference